anti- XRCC5/Ku80 antibody

There are at least two ways for eukaryotes to repair DNA double-strand breaks: homologous recombination and non-homologous end joining. The core mechanism of NHEJ includes XRCC4 (lupus autoantigen), DNA ligase IV and DNA-dependent protein kinase complex, the latter is composed of DNA ends bound to XRCC5 / XRCC6 heterodimer and catalytic subunit PRKDC. The heterodimer of XRCC5/XRCC6 increases the affinity of the catalytic subunit PRKDC to DNA by 100 times. Once the XRCC5/6 dimer associates with NAA15, it can bind to the osteocalcin promoter and activate osteocalcin expression. When used with APEX1, XRCC5/6 dimer can act as a negative regulator of transcription. Some published papers indicate that the molecular weight of XRCC5 is 86kDa, while more papers indicate that XRCC5 is an 80kDa protein because it was first introduced in a publication. Therefore, Ku80 and Ku86 are the same protein.