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| Catalog: | C-FC-0507A |
| Product Type: | FCM Antibody |
| Size: | 20 tests/50 tests/100 tests/200 tests |
| Reactivity: | Human |
| Analysis mode: | FCM |
| Host: | Mouse |
| Isotype: | Mouse IgG1, κ |
| Alternate names: | CD27L receptor,T-cell activation antigen CD27,T14,S152,TNFRSF7 |
| Form: | Liquid |
| Shipping: | Biological ice pack at 4℃ |
| Storage: | 2-8℃ |
| Conjugation: | APC,FITC,PerCP/Cyanine5.5 |
| Buffer: | PBS with 0.05% Proclin300, 1% BSA |
| Swiss port: | P26842 |
| Clone NO.: | OKT3,RPA-T4,OKT8 |
| Recommended use: | For whole blood samples, add 5 μL Anti-Human CD4+CD8α-Cyanine5.5 and other antibodies to 100 μL anticoagulant- treated blood sample. Mix and incubate the sample at RT in the dark for 15 min or at 2~8°C in the dark for 30 min. Remove red blood cells with RBC lysis solution following the manufacturer's instruction. Wash the cell pellet with cell staining buffer and discard the supernatant after centrifugation at 300 μg for 5 min. Resuspend the cells with 500 μL cell staining buffer and load the sample on flow cytometer for detection. For other samples, 1×105 ~108 dissociated single cells are centrifuged at 300 μg for 5 min with the supernatant discarded. Resuspend the cells with cell staining buffer and add 5 μL Anti-Human CD4+CD8α-Cyanine5.5 and other antibodies, making the final volume of reaction to 100 μL. Mix and incubate the sample at RT in the dark for 15 min or at 2~8°C in the dark for 30 min. Add 1~2 mL of cell staining buffer to each tube, centrifuge at 300 μg for 5~10 min and discard the supernatant. Resuspend the cells with 500 μL cell staining buffer and load the sample on flow cytometer for detection. It's recommended that the antibodies should be titrated to μget optimal results. |
This product is a FCM antibody cocktail made up of APC Anti-Human CD3 Antibody [Clone: OKT3] (Mouse IgG2a, κ), FITC Anti-Human CD4 Antibody [Clone: RPA-T4] (Mouse IgG1, κ) and PE/Cyanine5.5 Anti-Human CD8a Antibody [Clone: OKT-8] (Mouse IgG2a, κ).
CD3 is a heterotetrameric protein consisting of a CD3γ, a CDδ and 2 CD3ε. It forms complex with TCR. OKT-3 recognize human CD3ε. Human CD3 is expressed on the surface of T cells and NKT cells.
CD4 is also called Leu-3 or T4. It's a single-chain type I transmembrane glycoprotein, mainly expressed on the surface of T cells, and monocytes/macrophages. In T cells, CD4 forms complex with TCR/CD3 and play important roles in T cell immunity. The target of HIV is CD4+ T cells. Reduction of CD4+ T cells is the main reason of defected immunity after HIV infection.
CD8 is mainly expressed on cytotoxic T cells and also some subpopulations of NK cells. CD8 forms dimer function. In most cells, CD8 is a heterodimer consisting of CD8a and CD8b, but in NK cells nearly all CD8 is homodimer of CD8a. CD8a can form co-receptor with MHC-I restricted TCR to promote T cell antigen recognition and activation.
Core Diagnostic Capabilities
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