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| Catalog: | C-FC-0256A |
| Product Type: | FCM Antibody |
| Size: | 20 tests/50 tests/100 tests/500 tests |
| Reactivity: | Mouse |
| Analysis mode: | FCM |
| Host: | Rat |
| Isotype: | Rat IgG2a, κ |
| Alternate names: | Ly6a,Ly6,Ly-6A.2/Ly-6E.1,SCA-1,TAP |
| Form: | Liquid |
| Shipping: | Biological ice pack at 4℃ |
| Storage: | 2-8℃ |
| Conjugation: | AF488 |
| Buffer: | PBS with 0.05% Proclin300, 1% BSA |
| Swiss port: | P05533 |
| Clone NO.: | D7 |
| Recommended use: | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The amount of the reagent is suggested to be used 5 μL of antibody per test (million cells in 100 μL staining volume or per 100 μL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. |
Ly-6A/E, also known as Sca-1, is an 18 kD member of the Ly-6 multigene family. Ly6A/E is a glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA/2, SJL, SWR, and 129), Ly-6A/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB/c, CBA, C3H/He, DBA/1, and NZB) have low Ly-6A/E expression on resting peripheral lymphocytes. The expression of Ly-6A/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A/E is thought to be involved in the regulation of both T and B cell responses.
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