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| Catalog: | C-FC-0022A |
| Product Type: | FCM Antibody |
| Size: | 50 μg/500 μg/1 mg |
| Reactivity: | Human, Mouse |
| Analysis mode: | Depletion, Neut, ICFCM, WB, IHC |
| Host: | Rat |
| Isotype: | Rat IgG1, κ |
| Alternate names: | Interleukin-5,IL-5,B-cell differentiation factor I,T-cell replacing factor,TRF |
| Form: | Liquid |
| Shipping: | Biological ice pack at 4℃ |
| Storage: | 2-8℃ |
| Conjugation: | None (Purified antibody/Azide Free/Low endotoxin) |
| Buffer: | 0.2 μm filtered in PBS, pH7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or preservatives. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay. |
| Swiss port: | P05113,P04401 |
| Clone NO.: | TRFK5 |
| Recommended use: | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2 μg per 106 cells in 100 μL volume or 100 μL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
IL-5 is a homodimeric, disulphide-linked protein produced by T-cells. Monomeric human IL-5 is a 126 amino acid protein with a reported molecular weight of 26 kD for the homodimeric protein. Mouse and human IL-5 are approximately 70% identical. IL-5 has been shown to promote the growth of immature hematopoietic BFU-E progenitors, stimulate the activation and differentiation of eosinophils, and promote the generation of cytotoxic lymphocytes. Mouse IL-5 induces the differentiation and proliferation of pre-activated B-cells and stimulates the production and secretion of IgM and IgA by B-cells stimulated with bacterial endotoxin. The TRFK5 antibody can neutralize the bioactivity of natural or recombinant IL-5.
Core Diagnostic Capabilities
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